Investigation of Angiotensin-Convertings Enzyme Inhibitory Effects of Medicinal Plants Used in Traditional Persian Medicine for Treatment of Hypertension: Screening Study


Azita Kouchmeshky 1 , Seyed Behnamedin Jameie 4 , * , Gholamreza Amin 5 , Seyed Ali Ziai 6


1 Faculty of Pharmacy, Islamic Azad University, Tehran, IR Iran

4 Department of Anatomy, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, IR Iran

5 Department of Pharmacognosy, Faculty of Pharmacy, Tehran University, Tehran, IR Iran

6 Department of Pharmacology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IR Iran


Thrita: 1 (1); 13-23
Published Online: August 31, 2012
Article Type: Research Article
Received: September 16, 2011
Accepted: December 4, 2011




Background: Angiotensin converting enzyme (ACE) inhibitors are used widely in the treatment of hypertension and heart failure. These inhibitors such as captopril and enalapril are derived from natural products.

Objectives: The present study may represent an opportunity to find new non-chemical agents to develop new antihypertensive drugs.

Materials and Methods: In the present study 135 plants used in Traditional Persian Medicine have been investigated for their angiotensin converting enzyme (ACE) inhibitory activity. They were selected on the basis of their usage as antihypertensive, cardiotonics & diuretics. Dried powdered plant material was extracted with mix solution of water and ethanol in ultrasonic bath. The extracts were filtered and concentrated in vacuum except for the water extracts, which were freeze-dried. Test solutions were made by dissolving extract in assay buffer, corresponding to a final concentration of 0.33 mg of crude plant extract in 1 ml test volume. Enzyme assay was performed by HPLC method. Plants exhibiting inhibition levels with more than 50% were further tested for the presence of tannins in order to eliminate possible false positives.

Results: In total, 52 Species out of the 135 (39%) screened, gave more than 50% ACE inhibition. Forty Species were found to possess a high ACE inhibiting ability and were low in their tannin content.

Conclusions: Traditional medicine based on certain plants could be of beneficial effects in hypertension treatment.


Medicine, Traditional Hypertension Angiotensin-Converting Enzyme Inhibitors Tannins Plants, Medicinal

© 2012, Author(s). This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License ( which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
Full Text

Full text is available in PDF